Monday, November 22, 2010

How much buffer would I need to change a 750 mL volume of 10 mM pyruvic acid from pH 2.3 to 5.1?

A 50 mL sample of 10 mM pyruvic acid solution has a pH of 2.3. I used 2.1 mL of a phosphate buffer to bring this pH up to 5.1. How much buffer would I need to use for the same change in pH if I had a 750 mL sample of 10 mM pyruvic acid? Is it as simple as:



((2.1 mL buffer)*(750 mL pyruvic acid))/(50 mL pyruvic acid) = 31.5 mL buffer?How much buffer would I need to change a 750 mL volume of 10 mM pyruvic acid from pH 2.3 to 5.1?
Yes, you are absolutely right.

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